Expansion microscopy for Structural Cell Biology: from centriole architecture to retinopathies and associated gene therapies

Description

Abstract Ultrastructure expansion microscopy can be useful for structural cell biology, placing specific emphasis on centrioles and their inner scaffold structure. This discovery has significantly contributed to our comprehension of the molecular mechanisms underlying retinitis pigmentosa RP28 photoreceptor degeneration, thus paving the path for the development of a gene augmentation therapy strategy.

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The connecting cilium inner scaffold provides a structural foundation to maintain photoreceptor integrity. Mercey O, Kostic C, Bertiaux E, Giroud A, Morrison C., Sadian Y, Chang N, Arsenijevic Y, Guichard P** and Hamel V**. PLOS Biology, June 2022. https://doi.org/10.1371/journal.pbio.3001649

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Imaging cellular ultrastructures using expansion microscopy (U-ExM). Gambarotto D.##, Zwettler F. U. ##, Cernohorska M., Fortun D., Borgers S., Heine J., Schloetel J. G., Reuss M., Unser M., Boyden E.S., Sauer M**, Hamel V** and P. Guichard**. Nature Methods. January 2019. https://doi.org/10.1038/s41592-018-0238-1