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Striated Muscle Metabolic Cross-Talk in Health and Disease

Autophagy in pathophysiology

Chromatin dynamics, nuclear domains, virus

Patrick Lomonte

About

Beside the study of the biology of HSV-1, one of the main focus of the team is to understand, at the molecular level and using HSV-1 as a model, some basic aspects of nuclear and chromatin dynamics related to the maintenance of genome integrity and to the regulation of gene expression.

The major research interests of the team LOMONTE are:

The study of the pathological consequences of the infection of neurons from the peripheral and central nervous systems by the neurotropic virus herpes simplex virus 1 (HSV-1). We essentially focus our research on the establishment of latency and reactivation processes.
The study of the implication of nuclear architecture, especially promyelocytic leukemia nuclear bodies (PML NBs), in the regulation of latent HSV-1 chromatin features and in chromatin dynamics during the process of senescence.
The study of the chromatin alterations associated to damage or to genetic pathologies, and potentially linked to genetic instabilities, or motoneurons decay.

Members

Patrick Lomonte

Tristan Bargain

Pierre-Adrien Buffoni

Clinical Assistant Professor

Associate Professor

Clémence Guerriau

Research Assistant

Franceline Juillard

Assistant Professor

Research Assistant

Research Engineer

Delphine Poncet

Mazarine Couturier

Events

men and women sitting on chairs inside room

Nuclear dynamics of cGAS upon DNA-damage induced senescence

Florent Bressac

crowd of people sitting on chairs inside room

Probing cancer cell response upon mechanical or chemical stimuli

Charlotte Rivière

Abstract There are number of evidences indicating that both tumour micro-environment and mechanics are playing an important role in the […]

men and women sitting on chairs inside room

Implication of the SETDB1-HUSH-MORC2 axis in the control of herpes simplex virus 1 latency

The human body is colonized by numerous commensal microorganisms forming the human microbiota. The human microbiota is now recognized as […]

men and women sitting on chairs inside room

Role of PML nuclear bodies and of the histone chaperone complex HIRA in the regulation of chromatin dynamics

Constance Kleijwegt

Within the nucleus of eucaryotic cells, DNA is wrapped around histone proteins in order to form a structure called chromatin. […]

News

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Cristal Medal 2024 awarded to Karine Monier

Karine Monier, research engineer in the team Chromatin dynamics, nuclear domains, virus, has been awarded the Cristal medal from CNRS 2024 […]

Publications

Guerriau C, Léonce C, Carpentier C, Mokhtari K, Bielle F, Dridi-Aloulou A, Lomonte P, Meyronet D, S..., Functional analysis of telomere maintenance mechanisms is more informative than immunohistochemistry for ATRX mutation interpretation in Gliomas., Acta Neuropathol Commun 2025 Dec; 14(1): 25.

Florent Bressac, Tristan Bargain, Constance Kleijwegt, Wilhelm Bouchereau, Lucas Bonnefoy, Karine Mo..., A p97-PML NBs axis is essential for chromatin-bound cGAS untethering and degradation upon senescence-prone DNA damage, .

Escure T, Corpet A, Juillard F, Lomonte P, [Lethargy in progress! The HUSH complex controls HSV-1 latency]., Med Sci (Paris) 2025 Oct; 41(10): 737-740.

Lahaye X, Tran Van P, Chakraborty C, Shmakova A, Cao NTB, Ferran H, Ait-Mohamed O, Maurin M, Waterfa..., Centromeric DNA amplification triggered by viral proteins activates nuclear cGAS., Cell 2025 Jul; 188(15): 4043-4057.e21.

Wilhelm Bouchereau, Linda Chenane, Lola Lessard, Michèle Weiss-Gayet, Yseult Cardona, Rémi Mounier..., Defective plasticity in dermatomyositis patients muscle stem cells is associated with sustained intrinsic inflammatory signaling and disruption of the histone H3.3 chromatin loading pathway, .

Dutartre H, Fiorini F, Gerlier D, Gruffat H, Lomonte P, Lozach PY, Mateo M, [Lyon, at the center of the global virology research challenge]., Virologie (Montrouge) 2025 Apr; 29(2): 59-63.

Corentin Rousset, Rémi Neplaz, Pelin Catal, Elodie Chatre, Christophe Place, Patrick Lomonte, Franc..., Correlation-Based Nanometric Localization Using Lattice SIM² and dSTORM in PML Nuclear Bodies validated by Calibration Spheres, Biochem Biophys Res Commun 2025 Mar; 752(): 151450.

Simon Roubille, Tristan Escure, Franceline Juillard, Armelle Corpet, Remi Neplaz, Olivier Binda, Col..., The HUSH epigenetic repressor complex silences PML nuclear body- associated HSV-1 quiescent genomes, Proc Natl Acad Sci U S A 2024 Dec; 121(49): e2412258121.

Olivier Binda, Aimé Boris Kimenyi Ishimwe, Maxime Galloy, Karine Jacquet, Armelle Corpet, Amélie F..., The TUDOR domain of SMN is an H3K79 me1 histone mark reader, Life Sci Alliance 2023 Jun; 6(6): .

Constance Kleijwegt, Florent Bressac, Coline Seurre, Wilhelm Bouchereau, Camille Cohen, Pascale Texi..., Interplay between PML NBs and HIRA for H3.3 dynamics following type I interferon stimulus, Elife 2023 May; 12(): .

Simon Roubille, Franceline Juillard, Tristan Escure, Olivier Binda, Armelle Corpet, Stuart Bloor, Ca..., The HUSH-SETDB1-MORC2 epigenetic repressor complex restricts herpesvirus infection in association with PML nuclear bodies, .

Shaqraa Musawi, Lise-Marie Donnio, Zehui Zhao, Charlène Magnani, Phoebe Rassinoux, Olivier Binda, J..., Nucleolar reorganization after cellular stress is orchestrated by SMN shuttling between nuclear compartments, Nat Commun 2023 Nov; 14(1): 7384.

, Initial TK-deficient HSV-1 infection in the lip alters contralateral lip challenge immune dynamics, Sci Rep 2022 May; 12(1): 8489.

Olivier Binda, Franceline Juillard, Julia Novion Ducassou, Constance Kleijwegt, Geneviève Paris, An..., SMA-linked SMN mutants prevent phase separation properties and SMN interactions with FMRP family members, Life Science Alliance.

Arnaud Jacquier, Simon Roubille, Patrick Lomonte, Laurent Schaeffer, Microrchidia CW-Type Zinc Finger 2, a Chromatin Modifier in a Spectrum of Peripheral Neuropathies, Front Cell Neurosci 2022 ; 16(): 896854.

P. Billard, C. Guerriau, C. Carpentier, F. Juillard, N. Grandin, P. Lomonte, P. Kantapareddy, N. Duf..., The TeloDIAG: how telomeric parameters can help in glioma rapid diagnosis and liquid biopsy approaches, Annals of Oncology.

Armelle Corpet, Constance Kleijwegt, Simon Roubille, Franceline Juillard, Karine Jacquet, Pascale Te..., PML nuclear bodies and chromatin dynamics: catch me if you can!, Nucleic Acids Res 2020 Dec; 48(21): 11890-11912.

Camille Cohen, Armelle Corpet, Mohamed Ali Maroui, Franceline Juillard, Patrick Lomonte, Latent/Quiescent Herpes Simplex Virus 1 Genome Detection by Fluorescence In Situ Hybridization (FISH), .

Maria Elena Marcocci, Giorgia Napoletani, Virginia Protto, Olga Kolesova, Roberto Piacentini, Domeni..., Herpes Simplex Virus-1 in the Brain: The Dark Side of a Sneaky Infection, Trends Microbiol 2020 Oct; 28(10): 808-820.

Patrick Lomonte, Faouzi Baklouti, Olivier Binda, The Biochemistry of Survival Motor Neuron Protein Is Paving the Way to Novel Therapies for Spinal Muscle Atrophy, Biochemistry 2020 Apr; 59(14): 1391-1397.

Nathalie Grandin, Bruno Pereira, Camille Cohen, Pauline Billard, Caroline Dehais, Catherine Carpenti..., The level of activity of the alternative lengthening of telomeres correlates with patient age in IDH-mutant ATRX-loss-of-expression anaplastic astrocytomas, Acta Neuropathol Commun 2019 Nov; 7(1): 175.

Nolwenn Poccardi, Antoine Rousseau, Oscar Haigh, Julie Takissian, Thierry Naas, Claire Deback, Louis..., Herpes Simplex Virus 1 Replication, Ocular Disease, and Reactivations from Latency Are Restricted Unilaterally after Inoculation of Virus into the Lip, J Virol 2019 Dec; 93(24): .

Camille Cohen, Armelle Corpet, Simon Roubille, Mohamed Ali Maroui, Nolwenn Poccardi, Antoine Roussea..., Promyelocytic leukemia (PML) nuclear bodies (NBs) induce latent/quiescent HSV-1 genomes chromatinization through a PML NB/Histone H3.3/H3.3 Chaperone Axis, PLoS Pathog 2018 Sep; 14(9): e1007313.

Patrick Lomonte, Herpesvirus Latency: On the Importance of Positioning Oneself, Adv Anat Embryol Cell Biol 2017 ; 223(): 95-117.

Patrick Lomonte, The interaction between herpes simplex virus 1 genome and promyelocytic leukemia nuclear bodies (PML-NBs) as a hallmark of the entry in latency, Microb Cell 2016 Nov; 3(11): 569-572.

Adam S, Dabin J, Chevallier O, Leroy O, Baldeyron C, Corpet A, Lomonte P, Renaud O, Almouzni G, Polo..., Real-Time Tracking of Parental Histones Reveals Their Contribution to Chromatin Integrity Following DNA Damage., Mol Cell 2016 Oct; 64(1): 65-78.

Mohamed Ali Maroui, Aleth Callé, Camille Cohen, Nathalie Streichenberger, Pascale Texier, Julie Tak..., Latency Entry of Herpes Simplex Virus 1 Is Determined by the Interaction of Its Genome with the Nuclear Environment, PLoS Pathog 2016 Sep; 12(9): e1005834.

Chandran Ramakrishna, Adrianna Ferraioli, Aleth Calle, Thanh Nguyen, Harry Openshaw, Patric Lundberg..., Establishment of HSV1 Latency in Immunodeficient Mice Facilitates Efficient In Vivo Reactivation, PLoS Pathog 2015 Mar; 11(3): e1004730.

Frecha C, Chevalier SA, van Uden P, Rubio I, Siouda M, Saidj D, Cohen C, Lomonte P, Accardi R, Tomma..., Expression of the epidermodysplasia verruciformis-associated genes EVER1 and EVER2 is activated by exogenous DNA and inhibited by LMP1 oncoprotein from Epstein-Barr virus., J Virol 2015 Jan; 89(2): 1461-7.

S. Cavallero, N. Huot, L. Francelle, P. Lomonte, T. Naas, M. Labetoulle, Biological Features of Herpes Simplex Virus Type 1 Latency in Mice According to Experimental Conditions and Type of Neurones, Invest Ophthalmol Vis Sci 2014 Oct; 55(12): 7761-74.

Lomonte P, Corps nucléaires PML, centromères et contrôle de la latence du virus herpès simplex 1., Virologie (Montrouge) 2014 Jun; 18(3): 170-179.

Frédéric Catez, Antoine Rousseau, Marc Labetoulle, Patrick Lomonte, Detection of the Genome and Transcripts of a Persistent DNA Virus in Neuronal Tissues by Fluorescent In situ Hybridization Combined with Immunostaining, J Vis Exp 2014 Jan; (83): e51091.

M. Sabra, P. Texier, J. El Maalouf, P. Lomonte, The Tudor protein survival motor neuron (SMN) is a chromatin-binding protein that interacts with methylated lysine 79 of histone H3, J Cell Sci 2013 Aug; 126(Pt 16): 3664-77.

Frédéric Catez, Christel Picard, Kathrin Held, Sylvain Gross, Antoine Rousseau, Diethilde Theil, N..., HSV-1 genome subnuclear positioning and associations with host-cell PML-NBs and centromeres regulate LAT locus transcription during latency in neurons., PLoS Pathog 2012 ; 8(8): e1002852.

Sylvain Gross, Frédéric Catez, Hiroshi Masumoto, Patrick Lomonte, Centromere Architecture Breakdown Induced by the Viral E3 Ubiquitin Ligase ICP0 Protein of Herpes Simplex Virus Type 1., PLoS One 2012 ; 7(9): e44227.

Irena Draskovic, Nausica Arnoult, Villier Steiner, Silvia Bacchetti, Patrick Lomonte, Arturo Londoñ..., Probing PML body function in ALT cells reveals spatiotemporal requirements for telomere recombination, Proc Natl Acad Sci U S A 2009 Sep; 106(37): 15726-31.

Eric Morency, Mirna Sabra, Frédéric Catez, Pascale Texier, Patrick Lomonte, A novel cell response triggered by interphase centromere structural instability., J Cell Biol 2007 Jun; 177(5): 757-68.

Patrick Lomonte, Eric Morency, Centromeric protein CENP-B proteasomal degradation induced by the viral protein ICP0., FEBS Lett 2007 Feb; 581(4): 658-62.

P. Lomonte, ICP0 of herpes simplex virus type 1 : ubiquitination at the service of infection (review in French)., Virologie.

E. Morency, Y. Couté, J. Thomas, P. Texier, P. Lomonte, The protein ICP0 of herpes simplex virus type 1 is targeted to nucleoli of infected cells. Brief report., Archiv für die gesamte Virusforschung.

Delphine Cuchet, René Ferrera, Patrick Lomonte, Alberto L Epstein, Characterization of antiproliferative and cytotoxic properties of the HSV-1 immediate-early ICPo protein., J Gene Med 2005 Sep; 7(9): 1187-99.

E. Morency, Y. Coute, J. Thomas, P. Texier, P. Lomonte, The protein ICP0 of herpes simplex virus type 1 is targeted to nucleoli of infected cells., Archives of Virology.

D. Cuchet, R. Ferrera, P. Lomonte, Epstein A.L., Characterisation of the antiproliferative and cytotoxic properties of the HSV-1 immediate-early ICP0 protein., The Journal of Gene Medicine.

Patrick Lomonte, Joëlle Thomas, Pascale Texier, Cécile Caron, Saadi Khochbin, Alberto L Epstein, Functional interaction between class II histone deacetylases and ICP0 of herpes simplex virus type 1., J Virol 2004 Jul; 78(13): 6744-57.

P. Lomonte, J. Thomas, P. Texier, C. Caron, S. Khochbin, A. Epstein, Functional interaction between class II DDACs and ICP0 of herpes simplex virus type 1., Journal of Virology.

Patrick Lomonte, Kevin Sullivan, Roger Everett, Degradation of Nucleosome-associated Centromeric Histone H3-like Protein CENP-A Induced by Herpes Simplex Virus Type 1 Protein ICP0, Journal of Biological Chemistry.

P. Lomonte, Sullivan Kf, Everett Rd, Degradation of nucleosome-associated centromeric histone H3-like proein CENP-A induced by herpex simplex virus type 1 protein ICP0., Journal of Biological Chemistry.

A. Vanderplasschen, N. Markine-Goriaynoff, P. Lomonte, M. Suzuki, N. Hiraoka, J.-C. Yeh, F. Bureau, ..., A multipotential beta -1,6-N-acetylglucosaminyl-transferase is encoded by bovine herpesvirus type 4, Proceedings of the National Academy of Sciences of the United States of America.

A. Vanderplasschen, N. Markine-Goriaynoff, P. Lomonte, M. Suzuki, N. Hiraoka, Yeh Jc, F. Bureau, L. ..., a multipotential b-1,6-Nacetylglucosaminyltransferase is encoded by Bovine herpesvirus type 4., Proceedings of the National Academy of Sciences of the United States of America.

P. Lomonte, Everett Rd, Herpes simplex virus type 1 immediate-early protein Vmw110 inhibits progression of cells through mitosis and from G1 into S phase of the cell cycle., Journal of Virology.

Everett Rd, Earnshaw Wc, J. Findlay, P. Lomonte, Specific destruction of kietochore protein CENP-C and disrutpion of cell division by herpes simplex virus immediate-early protein Vmw110., EMBO Journal.

Everett Rd, P. Lomonte, T. Sternsdorf, Van Driel R, A. Orr, Cell cycle regulation of PML modification and ND10 composition., Journal of Cell Science.