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Published in: Biochem Biophys Res Commun 2025 Mar; 752(): 151450

Authors: Corentin Rousset, Rémi Neplaz, Pelin Catal, Elodie Chatre, Christophe Place, Patrick Lomonte, Franceline Juillard, Arnaud Favier, Karine Monier

Summary

We evaluated the Lattice di-SIM 3D structural illumination method with a focus on Promyelocytic Leukemia Nuclear Bodies (PML NBs). Lattice SIM 2 ‘s performance was compared to its predecessor using biological samples and calibration beads. Optimized SIM 2 parameters revealed PML NBs with a distinct ring-like morphology. Furthermore, we compared Lattice-SIM 2 with dSTORM, finding a strong correlation between blinking events and the SIM 2 mask. For 2D nanoscopic correlation, we used the long-lived imaging buffer Eternity, while the modified variant, Eternity-SIM, with a higher refractive index, provided improved 3D correlation for SIM 2 imaging.

Highlights:

 Optimized Lattice SIM 2 parameters to reveal the ring-like circular morphology of Promyelocytic Leukemia Nuclear Bodies (PML NBs)  Correlation of Lattice SIM 2 to Lattice SIM and to dSTORM imaging using biological samples and fluorescent spheres.  Investigated the effectiveness of Lattice SIM 2 to minimize Z-elongation compared to Lattice SIM  Correlative Lattice 3D SIM 2 -STORM nanoscopy with Eternity-SIM buffer to minimize refractive index differences.

Authorship: All authors should have made substantial contributions to all of the following:

(1) the conception and design of the study (KM, PL, FJ, AF, CP), Sample preparation (CR, RN, PC), acquisition of data (CR, RN, KM, EC), or analysis (CR, RN, KM), interpretation of data (CR, RN, KM, PL, FJ, CP) ;(2) drafting the article (CP, KM, FJ) or revising it critically for important intellectual content (CP, KM, PL, AF) ; (3) final approval of the version to be submitted (all).

Link to HAL – hal-04953475

Link to DOI – 10.1016/j.bbrc.2025.151450