Project: Mechanisms of membrane trafficking in differentiated cells

About

Secretory protein transport is necessary to fulfil essential cellular functions, enabling communication between cells, tissue maintenance and specialized functions. Differentiated tissues display distinct secretory needs, requiring adaptations of the transport routes and of the Golgi apparatus, the cellular central hub for secretion.Using human induced pluripotent stem cells (hiPSC)-derived models as well as primary cells, we explore the organization of the Golgi apparatus, the molecular players and the dynamics of protein transport using diverse approaches such as quantitative and real-time fluorescence microscopy, genome-editing and organelle-specific proteomics.Protein transport and intracellular organization are also modified in some diseases. Investigating the modification of protein transport routes and their dynamics in healthy and in cells bearing mutations is thus relevant to advance the understanding the pathogenesis of the disease but also to identify molecular levers, pavin